Rozlíšenie izomérnych zlúčenín pomocou vákuového UV detektora

Autoři

  • Ľ. Škultéty Mikrobiologický ústav AV ČR, Praha
  • T. Pluháček Mikrobiologický ústav AV ČR, Praha
  • V. Havlíček Mikrobiologický ústav AV ČR, Praha

Klíčová slova:

vákuový ultrafialový detektor, rozlíšenie, izomér, koelucia

Abstrakt

Although mass spectrometry has been utilized in a variety of applications, some limitations do exist where isomeric, isobaric, small, labile, or co-eluting compounds are analyzed. In this paper, a vacuum ultraviolet spectroscopy detector for gas chromatography (GC-VUV) is reviewed. The instrumentation is capable of quantitative and qualitative analysis of a wide variety of chemical compounds including hydrocarbons, permanent gases, fatty acids, pesticides, polychlorinated biphenyls, estrogens, explosives, psychoactive substances, and photoinitiators. Many chemical substances absorb UV radiation, have characteristic absorption cross-sections in the gas phase at a wavelength of 120–240 nm and can yield a unique absorption spectrum. Compound characterization thus can be accomplished by matching the captured VUV spectrum against the library of reference spectra. A potentially useful feature is the ability to deconvolute overlapping signals and to discriminate co-eluting analytes. If the VUV absorption cross-sections for chemical compounds are known, the accurate number of molecules in the detector (even without calibration) can be determined from the absorption signal.

Stahování

Publikováno

15.03.2020

Jak citovat

Škultéty, Ľ., Pluháček, T., & Havlíček, V. (2020). Rozlíšenie izomérnych zlúčenín pomocou vákuového UV detektora. Chemické Listy, 114(3), 230–238. Získáno z http://w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/3567

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