Hmotnostní spektrometrie s indukčně vázaným plazmatem – analýza jediné buňky

  • T. Pluháček Mikrobiologický ústav AV ČR, Praha
  • V. Maier Mikrobiologický ústav AV ČR, Praha
Klíčová slova: analýza jediné buňky, hmotnostní spektrometrie, hmotnostní spektrometrie s indukčně vázaným plazmatem

Abstrakt

The cell, as a fundamental structural, functional and biological unit, plays an essential role in living organisms. Analysis of the molecular/elemental composition of a single cell is a significant aspect of lipidomic, proteomic, metabolomic and metallomic studies aiming at understanding molecular processes in cells. Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful technique which can help to elucidate the bioeffects of trace metals and their species on cellular metabolism and cell behavior. Numerous ICP-MS-based methods have already been utilized to explore elemental/species profiles. These include, e.g. time-resolved ICP-MS, electrothermal vaporization ICP-MS, laser ablation ICP-MS, chip-based microextraction techniques, HPLC/CE-ICP-MS, elemental tagging, and mass cytometry. All these methods are covered in this short review.

Publikované
2020-03-15
Sekce
Články