Preparation of Polymer Immunoparticles as a Tool for Separation Oxidative Stress Biomarkers from Body Fluids

Authors

  • A. Némethová Department of Organic Technology, Institute of Chemical Technology, Prague
  • K. Syslová Department of Organic Technology, Institute of Chemical Technology, Prague
  • D. Pelclová Department of Occupational Medicine, 1st Faculty of Medicine, Charles University, Prague
  • P. Kačer Department of Organic Technology, Institute of Chemical Technology, Prague

Keywords:

polymeric immunoparticles, oxidative stress, 8-iso Prostaglandin F2α, LC-ESI-MS

Abstract

The work deals with preparation and characterization of polymer immunoparticles used as a separation tool for biomarkers from biological fluids like urine, plasma etc. Polymer imunoparticles consist of antibodies immobilized in a polyvinyl alcohol and polyethylene glycol matrix. Prepared immunoparticles, containing monoclonal antibodies against 8-iso Prostaglandin F2α, were used as a tool for a preliminary separation of 8-iso Prostaglandin F2α (a marker of oxidative stress) from complex biological matrix (urine) before a highly specific and precise detection and quantification by LC-ESI-MS method. The deve­loped method was characterized by high precision and accuracy. Functionality of the method was tested in a clinical study where urine concentration levels of 8-iso Prostaglandin F2α in patients with diagnosis of asbestosis or silicosis (oxidative stress induced diseases) and control group were compared.

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Published

2012-03-15

How to Cite

Némethová, A., Syslová, K., Pelclová, D., & Kačer, P. (2012). Preparation of Polymer Immunoparticles as a Tool for Separation Oxidative Stress Biomarkers from Body Fluids. Chemické Listy, 106(3), 183–188. Retrieved from http://w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/962

Issue

Vol. 106 No. 3 (2012)

Section

Articles