Utilization of Ultra Performance Liquid Chromatography (UPLC) – Modern Separation Technique for Determination of Vitamin E in Barley Cereals

Authors

  • K. Benešová Malting Institute Brno, Brno
  • H. Pluháčková Mendel University Brno
  • S. Běláková Malting Institute Brno, Brno
  • K. Vaculová Agricultural Research Institute, Kroměříž
  • R. Mikulíková Malting Institute Brno, Brno
  • J. Ehrenbergerová Mendel University Brno
  • N. Březinová Belcredi Mendel University Brno

Keywords:

UPLC, vitamin E, spring barley, tocopherols, tocotrienols

Abstract

A new UPLC method for the determination of vitamin E in barley cereals is proposed. The method shows higher sensitivity and lower limits of detection and quantification than the previous HPLC methods. The method was applied to various spring barley varieties. Although it was not possible to separate all vitamin E isomers due to the reverse phase used, the rapid and inexpensive method is suitable for a long-term monitoring of vitamin E in barley and other cereals.

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Published

2012-07-15

How to Cite

Benešová, K., Pluháčková, H., Běláková, S., Vaculová, K., Mikulíková, R., Ehrenbergerová, J., & Belcredi, N. B. (2012). Utilization of Ultra Performance Liquid Chromatography (UPLC) – Modern Separation Technique for Determination of Vitamin E in Barley Cereals. Chemické Listy, 106(7), 672–676. Retrieved from http://w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/895

Issue

Vol. 106 No. 7 (2012)

Section

Articles

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