Determination of Amphetamine and Methamphetamine And Their 4-Hydroxy Derivatives by HPLC with Fluorescence Detection

Authors

  • P. Pilařová Department of Pharmaceutical Chemistry and Drug Control, Charles University, Faculty of Pharmacy, Hradec Králové
  • P. Kastner Department of Pharmaceutical Chemistry and Drug Control, Charles University, Faculty of Pharmacy, Hradec Králové
  • J. Klimeš Department of Pharmaceutical Chemistry and Drug Control, Charles University, Faculty of Pharmacy, Hradec Králové

Keywords:

methamphetamine, fluorescence detection, dansyl chloride, HPLC, SPE

Abstract

A rapid, sensitive and low-cost method for the determination of methamphetamine and its metabolites in urine was developed. The tested substances were isolated by SPE, dansylated and analyzed by HPLC with fluorescence detection. The method was validated, the limit of detection for methamphetamine was lower than 1 ng ml–1; the limit of quantitation was 3 ng ml–1.

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Published

2013-01-15

How to Cite

Pilařová, P., Kastner, P., & Klimeš, J. (2013). Determination of Amphetamine and Methamphetamine And Their 4-Hydroxy Derivatives by HPLC with Fluorescence Detection. Chemické Listy, 107(1), 58–61. Retrieved from http://w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/789

Issue

Vol. 107 No. 1 (2013)

Section

Articles