Preparation of Base-Modified DNA by Enzymatic Incorporation of Modified Nucleoside Triphosphates

Authors

  • M. Hocek Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague | Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Prague

Keywords:

DNA, nucleotides, cross-coupling, polymerases

Abstract

Synthesis of base-modified oligonucleotides and DNA by polymerase incorporations of modified nucleoside triphosphates (dNTPs) is summarized. The cross-coupling synthesis of modified dNTPs, methods of their enzymatic incorporations, as well as their applications in bioanalysis and chemical biology are discussed.

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Published

2014-04-15

How to Cite

Hocek, M. (2014). Preparation of Base-Modified DNA by Enzymatic Incorporation of Modified Nucleoside Triphosphates. Chemické Listy, 108(4), 320–325. Retrieved from http://w.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/519

Issue

Vol. 108 No. 4 (2014)

Section

Articles