The Effect of Amphotericin B, Chitosan and Baicalein on Biofilm Formation of Trichosporon cutaneum CCY 30-5-10

Abstract

Trichosporon cutaneum is the second most significant opportunistic pathogen of genus Trichosporon after T. asahii. Its virulence is strongly tied to biofilm formation and colonization of various medical materials. Trichosporon spp. infections are ordinarily treated with antifungal azoles or polyene antibiotics. Nowadays, this type of therapy often fails and there is a great effort aimed at finding new antimicrobial substances, often originating from plant sources. These sources are frequently used as the components of traditional medicine.

This study is focused on the effect of antibiotic amphotericin B, polysaccharide chitosan, and flavonoid baicalein on the biofilm formation of T. cutaneum CCY 30-5-10. Their effects were studied by inverse microscope Cellavista using the image analysis evaluation of the area colonized by the biofilm in 96well microtitre plates. Baicalein was the most effective substance (concentration 40 mg L^‒1 caused 50% inhibition of cell adhesion and 75% eradication of mature biofilm). A significant effect was also observed when chitosan was applied (concentration 40 mg L^‒1 caused 69% inhibition of cell adhesion). Polyene antibiotic amphotericin B had distinctly stimulating effect on cell adhesion and only slightly eradicated the mature biofilm of T. cutaneum CCY 30-5-10.