Quantitative Analysis of Iodine Species in Biological Samples using Size Exclusion Chromatography and Inductively Coupled Plasma Mass Spectrometry

Abstract

The effect of nonspecific sorption of biomolecules in chromatographic columns and that of variable I contents in high-molecular-weight species on the determination of I species is illustrated by speciation analysis of animal tissues. I species were separated by SEC under the optimized conditions. The recoveries of the separations, limits of detection and limits of quantification were estimated.