Validácia stanovenia 1-hydroxypyrénu v moči metódou HPLC
Exposure to polycyclic aromatic hydrocarbons (PAH) is a serious hazard for human health. 1-Hydroxypyrene (1‑HOP), a major metabolite of pyrene, is used as a biomarker for the assessment of an exposure to PAH. The article deals with the validation of the HPLC determination of 1-HOP with fluorescence detection. 1-HOP was isolated from urine by solid phase extraction. The detection and quantification limits are 0.08 and 0.26 µg l–1, respectively. RSD of precision and accuracy are lower than 9.8 %. The recoveries are 78.8–93.5 %. This method can be used to determine and monitor the urinary 1-HOP in workers exposed to PAH.